|
Chikungunya Fever
|
0.030 |
Biomarker
|
disease |
BEFREE |
Our in-house RT-LAMP method and MinION sequencing system were also validated with RNAs and serum samples from recent outbreaks of CHIKV patients in Brazil.
|
31158221 |
2019 |
|
Chikungunya Fever
|
0.030 |
GeneticVariation
|
disease |
BEFREE |
Additionally, it explores the use of Nanopore metagenomic sequencing for DENV and CHIKV, which can likely be applied to other RNA viruses, highlighting the applicability of this approach to front-line public health and potential portable applications using the MinION.
|
30563591 |
2018 |
|
Chikungunya Fever
|
0.030 |
Biomarker
|
disease |
BEFREE |
We report unbiased metagenomic detection of chikungunya virus (CHIKV), Ebola virus (EBOV), and hepatitis C virus (HCV) from four human blood samples by MinION nanopore sequencing coupled to a newly developed, web-based pipeline for real-time bioinformatics analysis on a computational server or laptop (MetaPORE).
|
26416663 |
2015 |
|
Hepatitis C
|
0.020 |
Biomarker
|
disease |
BEFREE |
In conclusion, the MinION nanopore sequencer is useful for analyzing the HCV genome, especially the genomes of genotype 3 HCV strains for which standardized real- time PCR methods for all subgenotypes have not been established.
|
30924272 |
2019 |
|
Enterovirus Infections
|
0.020 |
Biomarker
|
group |
BEFREE |
Evaluation of MinION nanopore sequencing for rapid enterovirus genotyping.
|
29763627 |
2018 |
|
Malaria
|
0.020 |
Biomarker
|
disease |
BEFREE |
Here, we report the application of a portable sequencer, MinION, for genotyping the malaria parasite Plasmodium falciparum.
|
29844487 |
2018 |
|
Influenza A
|
0.020 |
Biomarker
|
disease |
BEFREE |
We designed an adapter to short highly conserved termini of the influenza A virus genome to target the (-) sense RNA into a protein nanopore on the Oxford Nanopore MinION sequencing platform.
|
30258076 |
2018 |
|
Influenza A
|
0.020 |
Biomarker
|
disease |
BEFREE |
Whole Genome Sequencing of Influenza A and B Viruses With the MinION Sequencer in the Clinical Setting: A Pilot Study.
|
30483243 |
2018 |
|
Enterovirus Infections
|
0.020 |
Biomarker
|
group |
BEFREE |
In this proof-of-concept study using Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16) strains as examples, we established an amplicon-based whole genome sequencing method using MinION.
|
29122092 |
2017 |
|
Malaria
|
0.020 |
Biomarker
|
disease |
BEFREE |
In this study, we demonstrate a new diagnostic procedure for human malaria using loop mediated isothermal amplification (LAMP) and the MinION™ nanopore sequencer.
|
28903726 |
2017 |
|
Hepatitis C
|
0.020 |
Biomarker
|
disease |
BEFREE |
We report unbiased metagenomic detection of chikungunya virus (CHIKV), Ebola virus (EBOV), and hepatitis C virus (HCV) from four human blood samples by MinION nanopore sequencing coupled to a newly developed, web-based pipeline for real-time bioinformatics analysis on a computational server or laptop (MetaPORE).
|
26416663 |
2015 |
|
Herpes Simplex Infections
|
0.010 |
Biomarker
|
group |
BEFREE |
In this work, we used the Sequel and MinION technique from PacBio and ONT, respectively, to characterize the lytic transcriptome of the herpes simplex virus type 1 (HSV-1).
|
31608102 |
2019 |
|
Virus Diseases
|
0.010 |
Biomarker
|
group |
BEFREE |
Acute Vector-Borne Viral Infection: Zika and MinION Surveillance.
|
31400093 |
2019 |
|
Meningitis, Bacterial
|
0.010 |
Biomarker
|
group |
BEFREE |
We investigated if the 16S amplicon sequencing performed by MinION, a nanopore sequencer, was capable of rapid pathogen identification in bacterial meningitis.
|
31444101 |
2019 |
|
Myelocerebellar Disorder
|
0.010 |
GeneticVariation
|
disease |
BEFREE |
Here we evaluate the potential of the Oxford Nanopore Technologies (ONT) MinION long-read sequencer for routine WGS by sequencing the reference sample NA12878 and the genome of an individual with ataxia-pancytopenia syndrome and severe immune dysregulation.
|
31015479 |
2019 |
|
Pneumocystis jiroveci pneumonia
|
0.010 |
Biomarker
|
disease |
BEFREE |
The aim of this study was to assess the ability of unbiased, genome-wide, long-read, shotgun sequencing using MinION to identify Pneumocystis jirovecii directly from respiratory tract specimens and to characterize the associated mycobiome.
|
31758176 |
2019 |
|
Multiple Chronic Conditions
|
0.010 |
Biomarker
|
disease |
BEFREE |
An integrated MinION analysis pipeline was assessed by performing WGS on 34 replicates across 3 multi-drug resistant isolates with known resistance mutations.
|
31234910 |
2019 |
|
Colorectal Carcinoma
|
0.010 |
Biomarker
|
disease |
BEFREE |
In summary, FusoPortal (http://fusoportal.org) is the first database of MinION-sequenced and completely assembled <i>Fusobacterium</i> genomes, and this central <i>Fusobacterium</i> genomic and bioinformatic resource will aid the scientific community in developing a deeper understanding of how this human pathogen contributes to an array of diseases, including periodontitis and colorectal cancer.<b>IMPORTANCE</b> In this report, we describe a hybrid MinION whole-genome sequencing pipeline and the genomic characteristics of the first eight <i>Fusobacterium</i> strains deposited in the FusoPortal database.
|
29976644 |
2018 |
|
Hepatitis B
|
0.010 |
Biomarker
|
disease |
BEFREE |
Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples.
|
29566006 |
2018 |
|
Influenza
|
0.010 |
Biomarker
|
disease |
BEFREE |
In this study, we developed a high-throughput WGS method for influenza viruses in clinical specimens with the MinION portable sequencer.
|
30483243 |
2018 |
|
Leishmaniasis, Cutaneous
|
0.010 |
Biomarker
|
disease |
BEFREE |
We diagnosed them as CL non-invasively by the direct boil loop-mediated isothermal amplification method and an innovative sequencing method using the MinION™ sequencer.
|
28288843 |
2018 |
|
Hairy Cell Leukemia
|
0.010 |
GeneticVariation
|
disease |
BEFREE |
In order to investigate the suitability of MinION sequencing on formalin-fixed paraffin-embedded samples, the presence and frequency of BRAF c.1799T > A mutation was investigated in two archival tissue specimens of Hairy cell leukemia and Hairy cell leukemia Variant.
|
29238890 |
2018 |
|
Myeloid Leukemia, Chronic
|
0.010 |
Biomarker
|
disease |
BEFREE |
FISH and MinION steps, respectively, together with ddPCR analysis, greatly reduce the complexity that has impeded the use of "personalized monitoring" of CML in clinical practice.
|
29541390 |
2018 |
|
Periodontitis
|
0.010 |
Biomarker
|
disease |
BEFREE |
In summary, FusoPortal (http://fusoportal.org) is the first database of MinION-sequenced and completely assembled <i>Fusobacterium</i> genomes, and this central <i>Fusobacterium</i> genomic and bioinformatic resource will aid the scientific community in developing a deeper understanding of how this human pathogen contributes to an array of diseases, including periodontitis and colorectal cancer.<b>IMPORTANCE</b> In this report, we describe a hybrid MinION whole-genome sequencing pipeline and the genomic characteristics of the first eight <i>Fusobacterium</i> strains deposited in the FusoPortal database.
|
29976644 |
2018 |
|
Tuberculosis
|
0.010 |
Biomarker
|
disease |
BEFREE |
MinION sequencing provided a fully annotated assembly of a transmissible XDR strain from an endemic setting and showed its utility to provide further understanding of genomic processes within Mycobacterium tuberculosis.
|
29906261 |
2018 |