There were no significant differences in the expressions of casein kinase 1 (CK1)‑ε, CK1γ or CK1δ; however, the mRNA and protein levels of CK1α were markedly higher in groups transfected with the T (those derived from the HCV‑J6 strain), NT (those derived from non‑tumor tissues) and C191 (those derived from tumor tissues) HCV core proteins than in mock group.
S238 is likely phosphorylated by casein kinase Iα, which requires a priming phosphorylation at S235.<b>IMPORTANCE</b> It has been known for years that the hepatitis C virus nonstructural protein 5A (NS5A) undergoes transition between two phosphorylation states: hypo- and hyperphosphorylation.
We also demonstrate the alpha isoform of Casein Kinase 1 (CK1alpha), a kinase previously identified as phosphorylating Hepatitis C Virus NS5A protein, also phosphorylates serine 56 of YFV methyltransferase.