|
Amyotrophic Lateral Sclerosis
|
0.020 |
GeneticVariation
|
disease |
BEFREE |
To elucidate the role of FUS in ALS, we overexpressed wild-type and two mutant forms of FUS in HEK-293T cells, as well as knocked-down FUS expression.
|
23577159 |
2013 |
|
Amyotrophic Lateral Sclerosis, Sporadic
|
0.010 |
GeneticVariation
|
disease |
BEFREE |
Genome-wide copy number variation in sporadic amyotrophic lateral sclerosis in the Turkish population: deletion of EPHA3 is a possible protective factor.
|
23991104 |
2013 |
|
Aortic Valve Insufficiency
|
0.010 |
AlteredExpression
|
disease |
BEFREE |
We determined mRNA and protein levels of EPHA3 and AR signaling‑related genes in the PCa cell line 22Rv1 by reverse transcription‑polymerase chain reaction (RT‑PCR) and western blotting, respectively.
|
29917167 |
2018 |
|
Asthma
|
0.010 |
Biomarker
|
disease |
BEFREE |
This study explores whether a chimeric gene R8, derived from the major group 1 allergen of house dust mite species (Dermatophagoides farinae and Dermatophagoides pteronyssinus), can be expressed in Human Embryonic Kidney 293 cells (HEK 293 T) and whether such a construct can be used as a DNA vaccine in asthma therapy.
|
25337189 |
2014 |
|
Atrial Fibrillation
|
0.400 |
Biomarker
|
disease |
CTD_human |
Biobank-driven genomic discovery yields new insight into atrial fibrillation biology.
|
30061737 |
2018 |
|
Atrial Fibrillation
|
0.400 |
Biomarker
|
disease |
CTD_human |
Multi-ethnic genome-wide association study for atrial fibrillation.
|
29892015 |
2018 |
|
Atrial Fibrillation
|
0.400 |
GeneticVariation
|
disease |
GWASCAT |
Biobank-driven genomic discovery yields new insight into atrial fibrillation biology.
|
30061737 |
2018 |
|
Atypical Ductal Breast Hyperplasia
|
0.020 |
GeneticVariation
|
disease |
BEFREE |
Expression of the N124K mutant receptor created by site-directed mutagenesis and transfected into HEK-293 cells was comparable with that of the wild-type (WT) receptor and two other mutant receptors N118K and L125P identified in subjects with ADH.
|
12162500 |
2002 |
|
Atypical Ductal Breast Hyperplasia
|
0.020 |
AlteredExpression
|
disease |
BEFREE |
All 4 known mutations causing BS type 5 and five ADH mutations were expressed in HEK 293T cells and receptor signalling was studied by measurement of intracellular free calcium in response to extracellular calcium ([Ca2+]o).
|
25506941 |
2014 |
|
Autosomal Recessive Polycystic Kidney Disease
|
0.010 |
Biomarker
|
disease |
BEFREE |
To determine whether defects in other human cystoproteins have similar effects, we studied extracellular acidification and glucose metabolism in human embryonic kidney (HEK-293) cell lines with polycystic kidney and hepatic disease 1 ( PKHD1) and polycystic kidney disease (PKD) 2 ( PKD2) truncating defects along multiple sites of truncating mutations found in patients with autosomal recessive and dominant PKDs.
|
30566001 |
2019 |
|
Bartter Disease
|
0.010 |
GeneticVariation
|
disease |
BEFREE |
All 4 known mutations causing BS type 5 and five ADH mutations were expressed in HEK 293T cells and receptor signalling was studied by measurement of intracellular free calcium in response to extracellular calcium ([Ca2+]o).
|
25506941 |
2014 |
|
Brain Damage, Chronic
|
0.010 |
AlteredExpression
|
disease |
BEFREE |
An additional 10% of the PAM group had an abnormally high activation ratio but normal ETK activity, as did 30% of the BDAM group.
|
12454526 |
2002 |
|
Breast adenocarcinoma
|
0.020 |
Biomarker
|
disease |
BEFREE |
Cytotoxicity studies on both cancerous (human breast adenocarcinoma MCF-7) and normal (Human embryonic kidney HEK-293) cell lines revealed their excellent biocompatibility.
|
29178994 |
2018 |
|
Breast adenocarcinoma
|
0.020 |
GeneticVariation
|
disease |
BEFREE |
Novel Mannich base derivatives of glabridin were synthesized and their antiproliferative activity were performed along with our previously reported glabridin-chalcone hybrids molecules (GCHMs) against various human cell lines MDA-MB-231 (breast adenocarcinoma), HEK-293 (embryonic kidney cell line), K562 (leukemia), MCF-7 (breast adenocarcinoma), HeLa (cervix adenocarcinoma), HepG2 (hepatocellular carcinoma) and WRL-68 (hepatic carcinoma).
|
30580626 |
2018 |
|
Breast Carcinoma
|
0.090 |
Biomarker
|
disease |
BEFREE |
In addition, cell-proliferation inhibition activity for all four ligands on the Human embryonic kidney (HEK-293) and breast cancer cell lines (MCF-7) was performed using MTT assay.
|
27748164 |
2017 |
|
Breast Carcinoma
|
0.090 |
Biomarker
|
disease |
BEFREE |
Transfection experiments which overexpressed the full-length EG-1 gene in human embryonic kidney HEK-293 cells or human breast cancer cell lines resulted in significantly increased in vitro proliferation, in comparison with transfection with empty vectors.
|
16024617 |
2005 |
|
Breast Carcinoma
|
0.090 |
Biomarker
|
disease |
BEFREE |
The in vitro biocompatibility and cytotoxicity tests of these films were also conducted using 3-(4,5-dimethylthiazole-2-yl-2,5-diphenyl tetrazolium bromide) assay of human embryonic kidney (HEK-293) and human breast cancer (MCF-7) cell lines up to 48 h, which shows their biocompatible nature.
|
30556014 |
2018 |
|
Breast Carcinoma
|
0.090 |
AlteredExpression
|
disease |
BEFREE |
And EPHA3 could be up-regulated by Sam68 in breast cancer.
|
31433759 |
2020 |
|
Breast Carcinoma
|
0.090 |
GeneticVariation
|
disease |
BEFREE |
In an effort to settle the controversy, we tested several stable E1A transfectants of cell lines MDA-MB-231, MCF-7, MDA-MB-435 (breast cancer), SKOV3-ipl (ovarian cancer), and PC-3 (prostate cancer), as well as parental and vector-transfected controls, HEK 293 cells, and RD-ES (Ewing sarcoma) cells, for the EWS-FLI1 fusion product.
|
11051226 |
2000 |
|
Breast Carcinoma
|
0.090 |
Biomarker
|
disease |
BEFREE |
We show that overexpression of JMJD6 increased HOTAIR expression and JMJD6 siRNAs suppressed it in ER+ MCF-7, triple negative MDA-MB-231 and non-breast cancer HEK 293 cells.
|
29229759 |
2018 |
|
Breast Carcinoma
|
0.090 |
Biomarker
|
disease |
BEFREE |
YH0618 selectively attenuated DOX-induced growth inhibition and apoptosis in human normal liver L02 cells and kidney HEK-293 cells, and simultaneously potentiated the anti-cancer effect of DOX in breast cancer MCF-7 and MDA-MB-231 cells by apoptosis pathways.
|
31333784 |
2019 |
|
Breast Carcinoma
|
0.090 |
Biomarker
|
disease |
BEFREE |
The proteins were added to human breast cancer cells (MDA-MB-231) and human embryonic kidney cells (HEK-293) in order to investigate the characteristic of selective targeting and releasing of scorpion toxin AGAP in cancer cells with high uPAR expression.
|
29172777 |
2017 |
|
Breast Carcinoma
|
0.090 |
Biomarker
|
disease |
BEFREE |
Based on these findings, we have synthesized some new diflunisal thiosemicarbazides and 1,2,4-triazoles and tested them against androgen-independent prostate adenocarcinoma (PC-3), colon carcinoma (HCT-116), human breast cancer (T47D), breast carcinoma (MCF7) and human embryonic kidney (HEK-293) cell lines.
|
30082676 |
2018 |
|
Brugada Syndrome (disorder)
|
0.010 |
GeneticVariation
|
disease |
BEFREE |
We report the analysis of two novel mutations on the same codon, Y1795C (LQT-3) and Y1795H (BrS), expressed in HEK 293 cells and characterized using whole-cell patch clamp procedures.
|
11410597 |
2001 |
|
Carcinogenesis
|
0.070 |
GeneticVariation
|
phenotype |
BEFREE |
Our study shows how biological complexity can challenge in vivo functional validation of mutations identified in sequencing efforts, and provides an incentive for the design of knock-in or conditional models to assign the role of EPHA3 mutation during lung tumorigenesis.
|
25713296 |
2015 |