|
Diabetes, Autoimmune
|
0.100 |
Biomarker
|
disease |
BEFREE |
HLA-DR3-DQ2<sup>+</sup>huCD4<sup>+</sup>IA/IE<sup>-/-</sup>RIP.B7.1<sup>+</sup> mice spontaneously developed autoimmune diabetes (incidence 46% by 35 weeks of age), accompanied by numerous hallmarks of human type 1 diabetes (autoantibodies against GAD65 and proinsulin; pancreatic islet infiltration by CD4<sup>+</sup>, CD8<sup>+</sup> B220<sup>+</sup>, CD11b<sup>+</sup> and CD11c<sup>+</sup> immune cells).
|
31612266 |
2019 |
|
Diabetes, Autoimmune
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
A list of 11 variables including clinical (gender, BMI, lipid profile, systolic and diastolic blood pressure and insulin-free time period), immunological (anti-GAD65, anti-IA-2 and anti-TPO antibody titers) and genetic features (predisposing gene variants previously identified as risk factors for autoimmune diabetes) retrieved from clinical records of 238 LADA patients referred to the Internal Medicine Unit of University of Sassari, Italy, were analyzed by PC analysis.
|
26612761 |
2016 |
|
Diabetes, Autoimmune
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Vaccination with a co-expression DNA plasmid containing GAD65 fragment gene and IL-10 gene induces regulatory CD4(+) T cells that prevent experimental autoimmune diabetes.
|
26797873 |
2016 |
|
Diabetes, Autoimmune
|
0.100 |
Biomarker
|
disease |
BEFREE |
We applied single-cell TCR α- and β-chain sequencing to peripheral blood GAD65-specific CD4(+) T cells, and TCR α-chain next-generation sequencing to bulk memory CD4(+) T cells to provide insight into TCR diversity in autoimmune diabetes mellitus.
|
25681349 |
2015 |
|
Diabetes, Autoimmune
|
0.100 |
Biomarker
|
disease |
BEFREE |
We focused here on generating an antigen-specific model of autoimmune diabetes in humanized double-transgenic mice carrying antigen-presenting HLA-DQ8 diabetes-linked haplotype and expressing human autoantigen GAD65 in pancreatic beta-cells using a relatively diabetes-susceptible strain of mice.
|
20350527 |
2010 |
|
Diabetes, Autoimmune
|
0.100 |
Biomarker
|
disease |
BEFREE |
A human T-cell receptor (TcR) derived from an autoreactive T-cell specific for GAD65, from a subject at high risk for autoimmune diabetes, was introduced into HLA-DR4 transgenic mice.
|
17949947 |
2008 |
|
Diabetes, Autoimmune
|
0.100 |
Biomarker
|
disease |
BEFREE |
CD4+ T cells specific for the diabetes-associated autoantigen GAD65 were analyzed using peripheral blood samples after pancreas transplantation in subjects with T1D with clinical evidence of recurrent autoimmune diabetes.
|
18455963 |
2008 |
|
Diabetes, Autoimmune
|
0.100 |
Biomarker
|
disease |
BEFREE |
Transgenic mice treated with GAD65 plasmid were protected from autoimmune diabetes, and had a lower number of CD4(+)hCD90(+) cells, confirming the pathogenic role of CD4(+)hCD90(+) cells in autoimmune diabetes.
|
16263243 |
2005 |
|
Diabetes, Autoimmune
|
0.100 |
Biomarker
|
disease |
BEFREE |
Antibodies to GAD65 and IA-2 are the major immunological markers in autoimmune diabetes.
|
12021128 |
2002 |
|
Diabetes, Autoimmune
|
0.100 |
Biomarker
|
disease |
BEFREE |
While both isoforms of glutamic acid decarboxylase (GAD) function as important autoantigens in autoimmune diabetes mellitus-GAD65 in humans and GAD67 in the NOD mouse-GAD67 is not synthesized in human pancreatic islets and is thought not to be an autoantigen in human diabetes.
|
12515288 |
2002 |
|
Diabetes, Autoimmune
|
0.100 |
Biomarker
|
disease |
BEFREE |
The major diabetes autoantigen, glutamic acid decarboxylase (GAD65), contains a region of sequence similarity, including six identical residues PEVKEK, to the P2C protein of coxsackie B virus, suggesting that cross-reactivity between coxsackie B virus and GAD65 can initiate autoimmune diabetes.
|
11034389 |
2000 |
|
Diabetes, Autoimmune
|
0.100 |
Biomarker
|
disease |
BEFREE |
To test the diagnostic sensitivity and specificity of epitopes of GAD65 autoantibodies (GAD65Ab) for insulin requirement, in patients with latent autoimmune diabetes of the adult, we studied 569 adult subjects with a clinical diagnosis of type 2 diabetes mellitus.
|
10634404 |
2000 |
|
Diabetes, Autoimmune
|
0.100 |
Biomarker
|
disease |
BEFREE |
The results suggest that GABA generated by either GAD65 or GAD67 is not critically involved in islet formation and that GAD65 expression is not an absolute requirement for development of autoimmune diabetes in the NOD mouse.
|
10422732 |
1999 |