Subgroup analysis revealed that there was a significant association between HSF1 overexpression and poor prognosis in esophageal squamous cell carcinoma (ESCC) (HR=1.83; 95% CI: 1.21-2.77; <i>P</i>=0.004), breast cancer (BC) (HR=1.52; 95% CI: 1.24-2.86; <i>P</i><0.001), hepatocellular carcinoma (HR=3.02; 95% CI: 1.77-5.18; <i>P</i><0.001), non-small-cell lung cancer (HR=2.19; 95% CI: 1.20-3.99; <i>P</i>=0.01), and pancreatic cancer (HR=2.58; 95% CI: 1.11-6.03; <i>P</i>=0.03) but not in osteosarcoma (HR=1.58; 95% CI: 0.47-5.35; <i>P</i>=0.46).
BACKGROUND The purpose of this study was to research the effects of hyperthermia on osteosarcoma (OS) by integrating the Chromatin Immunoprecipitation with the generation sequencing (ChIP-Seq) and TargetScan analysis of heat shock transcription factor 1 (HSF1).
Activation of the NF-κB pathway and expression of NF-κB-dependent genes were analyzed in TNFα-stimulated U-2 OS human osteosarcoma cells that were either heat-shocked or engineered to express a constitutively active form of HSF1 in the absence of heat shock.