insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Therefore, using time-resolved confocal fluorescence monitoring with MitoSOX Red, we investigated various effects of mitochondria-targeted antioxidants in model pancreatic <i>β</i>-cells (insulinoma INS-1E cells) and pancreatic islets.
|
31249641 |
2019 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Here, we describe experimental protocols to knock down β-arrestins by small interference RNA, to follow subcellular localization of β-arrestins in the cytosol and nucleus of the insulinoma INS-1E rat pancreatic β-cell line, and to analyze β-arrestin protein expression by Western blot using INS-1E cells and isolated mouse or human pancreatic islets.
|
30919365 |
2019 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Glucose stimulation consistently resulted in acidification of the matrix pH in INS-1E insulinoma cells and β-cells in intact human islets or islet monolayer cultures.
|
30476561 |
2019 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Effects on cell viability and apoptosis were assessed in insulinoma cell lines INS-1E (rat) and MIN6 (mouse) <i>in vitro</i> and were confirmed <i>in vivo</i> by using a mouse model of hepatic tumor dissemination after intrasplenic xenograft.
|
29051320 |
2018 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We investigated whether STZ reduced the production of intracellular insulin through autophagy in insulinoma INS-1E cells.
|
29485914 |
2018 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
To test the hypothesis that pro-inflammatory cytokines impair glucose-stimulated insulin secretion (GSIS) by inhibiting oxidative ATP synthesis, we probed insulin release and real-time mitochondrial respiration in rat INS-1E insulinoma cells that were exposed to a combination of 2 ng/mL interleukin-1-beta and 50 ng/mL interferon-gamma.
|
29953508 |
2018 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
While insulinomas and proinsulin-secreting tumors have many physiologic parallels, these cases illustrate several key distinctions in their diagnosis and management.
|
28510792 |
2017 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Unexpectedly, respiration in INS-1E insulinoma cells and primary human islets was unaltered in the presence of chloramphenicol.
|
28527697 |
2017 |
insulinoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The proinsulin levels and proinsulin/insulin molar ratio in patients with malignant versus benign insulinoma were 334 versus 44 pmol/L and 2.1 versus 0.9, respectively.
|
27984486 |
2017 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Furthermore, FGF21 was positively correlated with insulin (r = 0.80, P = 0.003) and proinsulin (r = 0.72, P = 0.012) in subjects with insulinoma.
|
28225059 |
2017 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
In insulinoma β-cells (INS-1E), Resv is previously shown to improve glucose-stimulated insulin secretion in a Sirt1-dependent mechanism and to protect against β-cell dedifferentiation in non-human primates, while inducing hypertrophy in myoblasts.
|
28108221 |
2017 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Knockdown (KD) of Klotho with siRNA in INS-1E insulinoma cells abrogated the protective effects of GABA against STZ toxicity.
|
28993191 |
2017 |
insulinoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
The read-through transcript INS-IGF2, composed of exons from the two genes proinsulin precursor (INS) and insulin‑like growth factor 2 (IGF2), both mapping to chromosomal subband 11p15.5, was highly expressed in the two insulinomas analyzed.
|
27667266 |
2016 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We have recently shown that palmitate-induced loss of INS-1E insulinoma cells is related to increased reactive oxygen species (ROS) production as both toxic effects are prevented by palmitoleate.
|
25482405 |
2015 |
insulinoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
To characterize these mutations, we transiently expressed proinsulin-GFP fusion proteins in MIN6 mouse insulinoma cells.
|
19952343 |
2010 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Measuring mitochondrial bioenergetics in INS-1E insulinoma cells.
|
19426881 |
2009 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
The present study provides evidence that chronically elevated concentrations of leptin and glucose induce beta-cell apoptosis through activation of the JNK pathway in human islets and in insulinoma (INS 832/13) cells.
|
18263705 |
2008 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Similarly transfected INS-1E insulinoma cells had diminished viability compared with those expressing WT proinsulin.
|
18451997 |
2008 |
insulinoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Small interfering RNA-mediated attenuation of SOX6 expression stimulated the proliferation of insulinoma INS-1E and NIH-3T3 cells, whereas retroviral overexpression resulted in inhibition of cell growth.
|
17412698 |
2007 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Intracellular degradation of the C-peptide of proinsulin, in a human insulinoma: identification of sites of cleavage and evidence for a role for cathepsin B.
|
7716141 |
1995 |
insulinoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Proinsulin radioimmunoassay in the evaluation of insulinomas and familial hyperproinsulinemia.
|
3023795 |
1986 |
insulinoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Much of the E1A-induced preproinsulin mRNA had a 5' end at the same position as the preproinsulin mRNA isolated from insulinoma cells, but a considerable fraction had 5' ends mapping heterogeneously within several hundred nucleotides of this site.
|
6199792 |
1984 |
insulinoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Thus, cell-free translation of human insulinoma mRNA yields an immunoreactive insulin larger than proinsulin, which is the same size as fish and rat preproinsulins recently described.
|
233702 |
1978 |