In addition, we found that the expression of LYN, an Src family tyrosine kinase, was inversely correlated with miR-122-5p expression in GC tissues by using western blot analysis, immunohistochemistry, and qRT-PCR assays.
Plasma <i>miR-122-5p</i> expression levels were also significantly higher in the AFPGC patients than the health volunteers and the non-AFPGC patients (<i>P</i> < 0.05) and were strongly correlated with plasma AFP levels (<i>r</i> = 0.7975, <i>P</i> < 0.0001).
In addition, cAMP responsive element binding protein 1 (CREB1) was identified as a direct target of miR-122, and its expression was negatively correlated with miR-122 expression in GC tissues (<i>r</i> = -0.711, <i>P</i> < 0.001).
Our results demonstrated that assessment of decreased circulating miR-122 and elevated circulating miR-192 levels has the potential to improve early detection of DM in GC.