|
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
In this study, we investigated whether NPY has a protective role in ER stress-induced neuronal cell death in SK-N-SH human neuroblastoma cells.
|
29650257 |
2018 |
|
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
A human neuroblastoma cell line (SH-SY5Y) was used to assess in vitro whether an AAV vector manufactured to clinical-grade protocols would be effective at transducing these cells to express NPY.
|
29367144 |
2018 |
|
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Our findings validate NPY as a therapeutic target for advanced NB and implicate the NPY/Y5R axis in disease dissemination.
|
27743558 |
2016 |
|
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Additionally, NPY and Y5R were upregulated in a BDNF-independent manner in NB cells under pro-apoptotic conditions, such as serum deprivation and chemotherapy, as well as in cell lines and tissues derived from posttreatment NB tumors.
|
25132261 |
2015 |
|
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Finally, we recapitulate our recent in-vitro evidence for the involvement of neurotrophin nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in the neuroprotective effect elicited by NPY in AD neuron-like models (neuroblastoma cells or primary cultures exposed to toxic concentrations of Aβ's pathogenic fragment 25-35), and propose a putative mechanism based on NPY-induced inhibition of voltage-dependent Ca(2+) influx in pre- and post-synaptic neurons.
|
25026432 |
2014 |
|
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
We have used human neuroblastoma SH-SY5Y cells to investigate the effect of valproate (VPA) and amitriptyline (AMI) on NPY expression at therapeutic plasma concentrations of 0.6mM and 630nM, respectively.
|
23994577 |
2013 |
|
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
NPY protected human SH-SY5Y dopaminergic neuroblastoma cells from 6-hydroxydopamine-induced toxicity.
|
21816512 |
2012 |
|
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Both NPY and Y2Rs are commonly expressed in neuroblastoma cell lines and tissues.
|
20676138 |
2010 |
|
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
These observations suggest that the expression of NPY in SH-SY5Y human neuroblastoma cells is negatively regulated by RA at the level of gene expression, probably by mechanisms involving the interaction of activated RARs with transcription factors (such as AP-1).
|
10854907 |
2000 |
|
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Glyceraldehyd-3-phospate dehydrogenase (GAPDH) and NPY gene expression in neuroblastomas of 50 patients were measured in parallel by competitive quantitative and TaqMan real-time RT-PCR.
|
11004664 |
2000 |
|
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
This occurred concomitant with induction of neurite outgrowth and expression of the neuronal marker genes GAP-43 and neuropeptide Y. Constitutive expression of exogenous HASH-1 did not alter the capacity of the neuroblastoma cells to differentiate in response to differentiation-inducing agents.
|
10080936 |
1999 |
|
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
In the present study, we have investigated whether these changes in CRE binding transcriptional factors activated by NPY results in gene regulation using a human neuroblastoma cell line (SK-N-BE2).
|
9802424 |
1998 |
|
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
In conclusion, the present study shows that the activation of PKC-coupled muscarinic receptors as the M3 subtype positively modulates the expression of the human NPY gene in SH-SY5Y neuroblastoma cells, via PKC-related mechanisms.
|
9666082 |
1998 |
|
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
NPY mRNA expression in neuroblastoma tissue was assessed by Northern blot analysis.
|
9802408 |
1998 |
|
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The suppression of neuropeptide Y Y1 receptor gene expression by antisense oligonucleotides targeted to different gene regions was monitored on mRNA and protein level in the human neuroblastoma SK-N-MC cell line.
|
9228417 |
1997 |
|
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Potassium currents were evoked by neuropeptide Y in oocytes injected with polyadenylated RNA or with cRNA from pools of a neuroblastoma (KAN-TS) cDNA library, and progressive subdivision of responding pools yielded a single cDNA.
|
8643076 |
1996 |
|
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Transfection of dividing neuroblastoma CHP126 cells with pJDT95npy resulted in the differential expression of chimeric NPY mRNAs derived from each promoter.
|
7968366 |
1994 |
|
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
These protein complexes appear to contribute to the cell specific expression of the NPY gene and seem to be required during differentiation of SH-SY5Y human neuroblastoma cells further along the sympathetic neuronal lineage induced by either TPA or staurosporine.
|
8036020 |
1994 |
|
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Activation of the human NPY gene during neuroblastoma cell differentiation: induced transcriptional activities of AP-1 and AP-2.
|
8123590 |
1994 |
|
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Thirty-eight neuroblastoma tumors were analyzed for NPY mRNA expression using in situ hybridization.
|
2393870 |
1990 |