Cell Counting Kit‑8, plate colony formation and Transwell assays were used to assess the effects of ACTL8 interference on the proliferation, migration and invasion of HNSCCPCI‑13 cells.
TEX produced by PCI-13 (HPV<sup>-</sup>) and UMSCC47 (HPV<sup>+</sup>) cell lines or from plasma of HNSCC patients were isolated by mini size exclusion chromatography (mini-SEC).
In the present study, the metastatic SCCHNPCI-37B cell line was utilized to explore the role of Janus activated kinase-3 (Jak3) in the CCR7-mediated signaling pathway in metastatic SCCHN cells.
Whereas growth inhibition of a breast carcinoma cell line induced by HSD17B12 knockdown was only reversed by AA, in a similar manner, the growth inhibition of the SCCHNPCI-13 cell line by HSD17B12 knockdown was reversed by E2 and AA.
In addition, when the ACT gene expression in PCI-04A cells was compared with the matched (from the same patient) metastatic HNSCC-derived cells (PCI-04B), increased steady-state level of the ACT mRNA was observed in PCI-04B cells.