|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
Very low levels of cyclin were observed in total human epidermis, and preliminary studies of two basaliomas have shown a significant synthesis of this protein in these tumors.
|
6203111 |
1984 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms.
|
1981239 |
1990 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Tumor labeling index (LI) was determined after in vitro 5-bromodeoxyuridine incorporation, while proliferating cell nuclear antigen LI was determined immunohistochemically.
|
1676611 |
1991 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
The percentage of tumour cells with positive staining for PCNA was found to correlate well with histological grading.
|
1680785 |
1991 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
Its frequency ranged from 0 to 80% (37.7 +/- 23.6) and larger sized, early-staged and DNA aneuploid tumors expressed a significant higher number of PCNA-reactive cells.
|
1361306 |
1992 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
The wide variation in PCNA expression between different samples within a tumour would indicate that systematic sampling and counting will be necessary in future immunohistochemical studies of cell proliferation in tumour material.
|
1351473 |
1992 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
The highest levels of PCNA were observed in advanced stage tumors with an amplified N-myc gene.
|
8093685 |
1993 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
These data show no significant correlation coefficient between Ki-67 or PCNA TPIs and SPFs derived from FCM analysis; however, the DNA diploid tumor subset categorized as having a greater than median SPF value had a significantly higher mean Ki-67 but not PCNA proliferation index.
|
8104388 |
1993 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Knowledge of the percentage of PCNA-positive cells could be especially helpful in deciding whether to treat patients with localized disease further because adjuvant chemotherapy affects mainly dividing cells and should, therefore, be more successful in tumors with high proliferative activity.
|
8095852 |
1993 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Various methods of measuring tumor cell proliferation (proliferating cell nuclear antigen, bromodeoxyuridine, Ki-67, nucleolar organizer regions, and DNA flow cytometry) are being compared with each other and correlated with clinical outcome in an attempt to validate these methods and to discover the most relevant one.
|
8494905 |
1993 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
In addition, using proliferating normal vs. human tumor breast cells as a model system, we observed a number of alterations in cyclin expression: (i) an 8-fold amplification of cyclin E gene in one tumor line, a 64-fold overexpression of its mRNA, and altered expression of its protein; (ii) deranged expression of cyclin E protein in all (10 of 10) tumor cell lines studied; (iii) increased cyclin mRNA stability, resulting in (iv) general overexpression of RNAs and proteins for cyclins A and B and CDC2 in 9 of 10 tumor lines and (v) deranged order of appearance of cyclins in synchronized tumor vs. normal cells, with mitotic cyclins appearing prior to G1 cyclins.
|
8430082 |
1993 |
|
Neoplasms
|
0.100 |
GeneticVariation
|
group |
BEFREE |
There was a weak correlation between the Ki67 and the PCNA indices (r = 0.38 p < 0.005), but no correlation was found between either index and oestrogen receptor status, patient age or tumour size.
|
7903289 |
1993 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
There was no linear correlation between numbers of ISH signals, PCNA positivity and tumor stages, and keratinization of squamous cell lung cancer.
|
7911024 |
1993 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Results of quantitative DNA measurements and PCNA scores were compared to clinical symptoms, histology, and time between first onset of symptoms and diagnosis of the tumor.
|
7904813 |
1994 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Tumor and nontumor PCNA (T-PCNA and NT-PCNA) scores were assessed by counting the positive staining nuclei per 1000 cells.
|
7513246 |
1994 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Morphometric, DNA, and proliferating cell nuclear antigen (PCNA) measurements were taken of benign melanocytic tumors and malignant melanomas.
|
7864299 |
1994 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Relatively larger diameter tumors (more than 5cm), poorly differentiated tumors, tumors with distant lymph node metastasis, and DNA aneuploidy tumors were positive for p53 more frequently. p53-positive cases had a higher PCNA labeling index and a poorer prognosis than p53-negative cases.
|
7915093 |
1994 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
The logistic regression analysis identified PCNA labeling rates (LRs), tumor size, and macroscopic type as independent significant factors for lymph node metastasis.
|
7910957 |
1994 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
We analyzed primary tumor tissue from 89 clinical stage I nonseminomatous germ cell testicular tumor patients for proliferating cell nuclear antigen expression and histological features to determine if these elements could distinguish pathological stage I (52 patients) from pathological stage II disease or patients who later had relapse (37).
|
7915335 |
1994 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
The results were correlated with the outcome, with the expression of proliferating cell nuclear antigen (PCNA)/cyclin (as a marker of cell proliferation) and with other well established prognostic factors for PTC (age, grading, extension and tumour size; Endocrinol Metab Clin North Am 1990, 19, 545-576). p21 staining in tumours of living patients was negative in 15, weakly positive (1+) in 10 and strongly positive (2+ or more) in 6 patients.
|
7908818 |
1994 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Overall, the PCNA and Ki67 labelling index showed a similar distribution pattern in normal and tumor tissue.
|
7517683 |
1994 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
The hypothesis that alterations in tumor suppressor gene expression was related to cell proliferation was tested by simultaneously demonstrating the expression of the proliferating cell nuclear antigen in methacarn-fixed, paraffin-embedded tissue sections of the same tumors.
|
8552572 |
1995 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
DNA ploidy and tumor proliferative activity (derived from proliferating cell nuclear antigen, PCNA FCM expression) were evaluated.
|
7571041 |
1995 |
|
Neoplasms
|
0.100 |
GeneticVariation
|
group |
BEFREE |
Tumors with a PCNA index < 6.056% showed a diameter less than the mean tumoral diameter and a benign clinical course.
|
7745970 |
1995 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
In conclusion, the overlapping expression patterns of PCNA and p53 in keratocanthoma-like tumors support the hypothesis that these tumors represent a possible biologic spectrum.
|
8600793 |
1995 |