We examined the effectiveness of 20× concentrated supernatant from <i>Lactobacillus gasseri</i> strain 63 AM (LgCS) grown in de Man, Rogosa and Sharpe broth in inhibiting <i>P. aeruginosa</i> biofilms <i>in vitro</i>, as well as in reducing wound bioburden and <i>P. aeruginosa</i> sepsis <i>in vivo</i>.<b>Results</b>.LgCS inhibited the growth of <i>P. aeruginosa</i> strain PAO1, prevented its biofilm development and eliminated partially developed PAO1 biofilms.
Mutation of the ybtQ homolog in P. aeruginosa strain PA14 significantly attenuates the virulence of this strain in both G. mellonella and a burned mouse model of sepsis to levels comparable to those seen with PAO1.
We found that P. aeruginosa PAO700 (algR::Gm(r)) was less lethal than PAO1, as tested in an acute septicemia infection mouse model, and was cleared more efficiently in a mouse pneumonia model.