We examined the effectiveness of 20× concentrated supernatant from <i>Lactobacillus gasseri</i> strain 63 AM (LgCS) grown in de Man, Rogosa and Sharpe broth in inhibiting <i>P. aeruginosa</i> biofilms <i>in vitro</i>, as well as in reducing wound bioburden and <i>P. aeruginosa</i> sepsis <i>in vivo</i>.<b>Results</b>.LgCS inhibited the growth of <i>P. aeruginosa</i> strain PAO1, prevented its biofilm development and eliminated partially developed PAO1 biofilms.
We found that P. aeruginosa PAO700 (algR::Gm(r)) was less lethal than PAO1, as tested in an acute septicemia infection mouse model, and was cleared more efficiently in a mouse pneumonia model.
Mutation of the ybtQ homolog in P. aeruginosa strain PA14 significantly attenuates the virulence of this strain in both G. mellonella and a burned mouse model of sepsis to levels comparable to those seen with PAO1.