We conclude that Rag1 <sup>-/-</sup> mice are an appropriate and novel xenograft model for studying the relationship between cancer and hyperinsulinaemia.
The V(D)J recombination of IgH and IgL loci, the Sgamma1/2-Smu switch circle, and the expression of RAG1 and RAG2 were also found in these cancer cell lines.
Both RAG1 and RAG2 were expressed in these human epithelia-derived cancer cell lines and the sequence was identical to that expressed in pre-B and pre-T cells.
To assess the effect of the difference in cancer cell types on the recruitment of BMD-VE and BMD-MF, 10 kinds of human cancer cell line were implanted into the subcutaneous tissue of the immunodeficient mice transplanted with bone marrow of double-mutant mice (RAG-1-/- beta-gal Tg or RAG-1-/- GFP Tg).