Neuroblastoma
|
0.400 |
Biomarker
|
disease |
BEFREE |
Here, we investigated the role of the UPS and autophagy by applying the potassium ionophore Valinomycin in PINK1-deficient human fibroblasts and isogenic neuroblastoma cell lines generated by CRISPR/Cas9.
|
30375512 |
2019 |
Neuroblastoma
|
0.400 |
Biomarker
|
disease |
BEFREE |
We used differentiated human dopaminergic neuroblastoma cell lines with stable PINK1 or DJ-1 knockdown to study live motility of mitochondria in neurites.
|
30133157 |
2018 |
Neuroblastoma
|
0.400 |
Biomarker
|
disease |
BEFREE |
These abnormalities are associated with increased inflammation-induced NF-κB signaling in astrocytes, and cause enhanced death of neurons co-cultured with inflamed PINK1 <sup>-/-</sup> mixed glia and neuroblastoma cells exposed to conditioned medium from LPS/IFN-γ treated PINK1 <sup>-/-</sup> mixed glia.
|
29321620 |
2018 |
Neuroblastoma
|
0.400 |
Biomarker
|
disease |
BEFREE |
The aim of the present study was to create a cellular model of PD using siRNA-mediated knock down of PINK1 in SH-SY5Y neuroblastoma cells The possible protective effects of curcumin, known for its many beneficial properties including antioxidant and anti-inflammatory effects, was tested on this model in the presence and absence of paraquat, an additional stressor.
|
27003823 |
2017 |
Neuroblastoma
|
0.400 |
GeneticVariation
|
disease |
BEFREE |
Marked dysregulations of microbial defense factors Ifit3 and Rsad2 were consistently observed upon five analyses: (1) Pink1 <sup>-/-</sup> primary neurons in the first weeks after brain dissociation, (2) aged Pink1 <sup>-/-</sup> midbrain with transgenic A53T-alpha-synuclein overexpression, (3) human neuroblastoma cells with PINK1-knockdown and murine Pink1 <sup>-/-</sup> embryonal fibroblasts undergoing acute starvation, (4) triggering mitophagy in these cells with trifluoromethoxy carbonylcyanide phenylhydrazone (FCCP), and (5) subjecting them to pathogenic RNA-analogue poly(I:C).
|
28768533 |
2017 |
Neuroblastoma
|
0.400 |
AlteredExpression
|
disease |
BEFREE |
In this study, we report that increased mitochondrial PINK1 levels observed in human neuroblastoma SH-SY5Y cells after carbonyl cyanide m-chlorophelyhydrazone (CCCP) treatment were due to de novo protein synthesis, and not just increased stabilization of full length PINK1 (FL-PINK1).
|
24184327 |
2014 |
Neuroblastoma
|
0.400 |
Biomarker
|
disease |
BEFREE |
Here, we demonstrate that Miro1, an outer mitochondrial membrane (OMM) protein crucial for the regulation of mitochondrial trafficking and distribution, is a substrate of the PINK1/Parkin mitochondrial quality control system in human dopaminergic neuroblastoma cells.
|
24671417 |
2014 |
Neuroblastoma
|
0.400 |
Biomarker
|
disease |
CTD_human |
The genetic landscape of high-risk neuroblastoma.
|
23334666 |
2013 |
Neuroblastoma
|
0.400 |
AlteredExpression
|
disease |
BEFREE |
We previously reported that stable neuroblastoma SH-SY5Y cell lines with reduced expression of endogenous PINK1 exhibit mitochondrial fragmentation, increased mitochondria-derived superoxide, induction of compensatory macroautophagy/mitophagy and a low level of ongoing cell death.
|
21637291 |
2011 |
Neuroblastoma
|
0.400 |
GeneticVariation
|
disease |
BEFREE |
To investigate the cytoprotective physiological function of PINK1, we used primary fibroblasts from three patients homozygous for G309D-PINK1 as well as SHEP neuroblastoma cells stably overexpressing GFP-tagged wild type (wt) PINK1.
|
20045449 |
2010 |
Neuroblastoma
|
0.400 |
Biomarker
|
disease |
BEFREE |
Here, we show that Parkin, PINK1, and DJ-1 formed a complex (termed PPD complex) to promote ubiquitination and degradation of Parkin substrates, including Parkin itself and Synphilin-1 in neuroblastoma cells and human brain lysates.
|
19229105 |
2009 |
Neuroblastoma
|
0.400 |
PosttranslationalModification
|
disease |
BEFREE |
Indeed, overexpression of PINK1 protects neuroblastoma cells from undergoing neurotoxin-induced apoptosis.
|
17000703 |
2006 |