Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Real-time determination of aggregated alpha-synuclein induced membrane disruption at neuroblastoma cells using scanning ion conductance microscopy.
|
29974096 |
2018 |
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Different NACP-Rep1 alleles have been associated with sporadic Parkinson's disease in some, but not all, studies and can effect expression driven by the SNCA promoter over a three-fold range in the neuroblastoma cell line, SH-SY5Y.
|
12923682 |
2003 |
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Likewise, in human neuroblastoma SH-SY5Y cells exposed to low-dose MPP<sup>+</sup> , Nramp1 expression and cathepsin D activity were decreased, along with an increase in α-synuclein protein expression and aggregation.
|
29679389 |
2019 |
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
In this study, we found that loss of GBA function resulted in increased levels of SNCA via inhibition of the autophagic pathway in SK-N-SH neuroblastoma cells, primary rat cortical neurons, or the rat striatum.
|
26378614 |
2015 |
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Human dopaminergic neuroblastoma SH-SY5Y cells were stably transduced with various isoforms of alpha-synuclein and their survival following exposure to hydrogen peroxide or to the dopaminergic neurotoxin MPP(+) was assessed.
|
10799759 |
2000 |
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Fe(II)-induced DNA damage in alpha-synuclein-transfected human dopaminergic BE(2)-M17 neuroblastoma cells: detection by the Comet assay.
|
14535945 |
2003 |
Neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
We investigated transcriptional changes in neuroblastoma cell lines transfected with either normal or mutant (A30P or A53T) alpha-synuclein using microarrays, with confirmation of selected genes by quantitative RT-PCR.
|
12716427 |
2003 |
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
In the present study we tested whether presence of human mutant GCase leads to accumulation and aggregation of α-synuclein in two models: in SHSY5Y neuroblastoma cells endogenously expressing α-synuclein and stably transfected with human GCase variants, and in Drosophila melanogaster co-expressing normal human α-synuclein and mutant human GCase.
|
30615125 |
2019 |
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
To explore CpG methylation associating with α-synuclein transcription and its underlying mechanisms in the neurotoxin-induced PD pathology, human neuroblastoma SH-SY5Y cells were treated with neurotoxins 6-hydroxydopamine (6-OHDA) and 1-methyl-4-phenylpyridinium (MPP<sup>+</sup>).
|
28807729 |
2017 |
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
C/EBPβ overexpression increases SNCA expression in neuroblastoma cells and putative C/EBPβ and δ binding sites are present in the SNCA genomic region suggesting that these proteins could regulate SNCA transcription.
|
31209363 |
2020 |
Neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
The Parkinson disease-associated A30P mutation stabilizes alpha-synuclein against proteasomal degradation triggered by heme oxygenase-1 over-expression in human neuroblastoma cells.
|
19457084 |
2009 |
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Retinoic acid promotes differentiation and α-synuclein oligomer formation in neuroblastoma cells, while addition of a proteasomal inhibitor induces neurite outgrowth and toxicity to certain wild-type and truncated α-synuclein clones.
|
21848810 |
2011 |
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
SK-N-MC neuroblastoma cells stably expressing the human dopamine transporter were transfected with human alpha-synuclein and cell clones with and without alpha-synuclein immunoreactivity were obtained.
|
14698476 |
2004 |
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Herein we have investigated the perturbations induced by low molecular weight α-synuclein and different types of α-synuclein oligomers in the neuroblastoma SH-SY5Y cells.
|
29539261 |
2018 |
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Dual effects of alpha-synuclein on neurotoxicity induced by low dosage of rotenone are dependent on exposure time in dopaminergic neuroblastoma cells.
|
20596942 |
2010 |
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
We found that oligomeric αSyn increased intracellular Ca(2+) levels, induced calcineurin (CaN) activity, decreased cAMP response element-binding protein (CREB) transcriptional activity and resulted in calcineurin-dependent death of human neuroblastoma cells.
|
22060133 |
2012 |
Neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
TH1-S31E associated with vesicular monoamine transporter 2 (VMAT2) and α-synuclein in neuroblastoma cells, and endogenous THSer(P)-31 was detected in VMAT2- and α-synuclein-immunoprecipitated mouse brain samples.
|
28637871 |
2017 |
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Misfolded α-synuclein causes hyperactive respiration without functional deficit in live neuroblastoma cells.
|
31848207 |
2020 |
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
RNA interference-mediated knockdown of alpha-synuclein protects human dopaminergic neuroblastoma cells from MPP(+) toxicity and reduces dopamine transport.
|
17131421 |
2007 |
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
The present study determined the impact of developmental Pb exposure on the α-Syn pathways in a mouse model knock-out (KO) for murine tau gene and in differentiated human neuroblastoma SHSY5Y cell line exposed to a series of Pb concentrations.
|
30068273 |
2018 |
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Differential cytotoxicity of human wild type and mutant alpha-synuclein in human neuroblastoma SH-SY5Y cells in the presence of dopamine.
|
15122920 |
2004 |
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
In support of these conclusions, mutations that target the region that promotes strong membrane interactions by α-synuclein oligomers suppressed their toxicity in neuroblastoma cells and primary cortical neurons.
|
29242346 |
2017 |
Neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Marked dysregulations of microbial defense factors Ifit3 and Rsad2 were consistently observed upon five analyses: (1) Pink1 <sup>-/-</sup> primary neurons in the first weeks after brain dissociation, (2) aged Pink1 <sup>-/-</sup> midbrain with transgenic A53T-alpha-synuclein overexpression, (3) human neuroblastoma cells with PINK1-knockdown and murine Pink1 <sup>-/-</sup> embryonal fibroblasts undergoing acute starvation, (4) triggering mitophagy in these cells with trifluoromethoxy carbonylcyanide phenylhydrazone (FCCP), and (5) subjecting them to pathogenic RNA-analogue poly(I:C).
|
28768533 |
2017 |
Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Propanoylated lysine, a specific indicator of docosahexaenoic acid oxidation, was increased in neuronal differentiated human neuroblastoma SH-SY5Y cells overexpressing α-synuclein.
|
29892158 |
2018 |
Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We also show that squalamine almost completely suppresses the toxicity of α-synuclein oligomers in human neuroblastoma cells by inhibiting their interactions with lipid membranes.
|
28096355 |
2017 |