Moreover, STK39 knockdown in osteosarcoma cells significantly affected the expression of proteins connected to cell proliferation (proliferating cell nuclear antigen and p21) and invasion [Twist1, matrix metalloproteinase (MMP)2 and MMP9].
We have demonstrated in this study that miR-33a is up-regulated in chemoresistant OS and that the miR-33a level is negatively correlated with the TWIST protein level in OS.
This study provides the first evidence of a functional link between TWIST and β-catenin signaling in osteosarcoma cells, which adds fresh insights into the molecular mechanism of osteosarcoma development.