|
Microphthalmos
|
0.100 |
Biomarker
|
disease |
BEFREE |
In addition, quantitative real-time PCR (qPCR) and Western blot experiments were performed to determine the expression of essential melanogenesis-related proteins including tyrosinase (TYR), tyrosinase-related protein (TRP) 1 and 2 and microphthalmia transcription factor (MITF).
|
31785464 |
2020 |
|
Microphthalmos
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Although phenylethyl resorcinol decreased mRNA levels of tyrosinase and tyrosinase-related protein (TRP)-2, it did not affect mRNA levels of melanogenic gene microphthalmia-associated transcriptional factor (MITF) or TRP-1.
|
30849679 |
2019 |
|
Microphthalmos
|
0.100 |
Biomarker
|
disease |
BEFREE |
STTM‑miR‑143‑5p overexpression resulted in an increased expression of downstream melanogenesis genes including microphthalmia‑associated transcription factor (MITF), tyrosinase family members [tyrosinase (TYR) and tyrosinase‑related protein 1 (TYRP1)], melanophilin (MLPH), and Rab27a, thereby contributing to melanocyte pigmentation by promoting total alkali‑soluble melanogenesis (ASM) and eumelanin (EM) contents; conversely, STTM‑miR‑143‑5p overexpression resulted in decreased expression of the tyrosinase‑related protein 2 (TYRP2)/dopachrome tautomerase (DCT), which is responsible for decreased pheomelanin (PM) content in mouse melanocytes.
|
31322203 |
2019 |
|
Microphthalmos
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
In addition, resorcinol suppressed the expression of melanogenic gene microphthalmia-associated transcriptional factor (MITF) and its downstream target genes tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2.
|
29621941 |
2018 |
|
Microphthalmos
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Ferulic acid inhibited melanin synthesis, tyrosinase expression, and microphthalmia transcription factor expression in B16F10 cells stimulated with α-melanocyte stimulating hormone.
|
28884442 |
2018 |
|
Microphthalmos
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The effects of diethylstilbestrol on cell viability, melanin content, tyrosinase activity, cAMP level, expression of the tyrosinase family and microphthalmia related transcription factor (MITF) were measured in B16 melanoma.
|
21745488 |
2011 |
|
Microphthalmos
|
0.100 |
Biomarker
|
disease |
BEFREE |
Included in these measurements were tyrosinase and microphthalmia transcription factor (MITF) protein levels, cyclic AMP levels, protein kinase A (PKA) activation, and reduced glutathione (GSH) and oxidized glutathione (GSSG) levels.
|
16394501 |
2006 |
|
Microphthalmos
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The transcription activation domain of microphthalmia transcription factor, tested as a GAL-MITF fusion protein, remained fully functional in these cells, however, and ectopic microphthalmia transcription factor localized normally to the nucleus and bound to the tyrosinase initiator E-box in gel retardation assays.
|
11886515 |
2001 |
|
Microphthalmos
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The authors tested 15 AMLs with T311 and D5 by immunohistochemistry and a subset of 3 cases by reverse transcription-polymerase chain reaction for their expression of tyrosinase and Mitf mRNA.
|
11277411 |
2001 |
|
Microphthalmos
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Recently, we have shown that Microphthalmia mediates the melanogenic effects elicited by alphaMSH that up-regulates the expression of tyrosinase through the activation of the cAMP pathway.
|
10707962 |
2000 |
|
Microphthalmos
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
These findings disclose the mechanism by which cAMP stimulates tyrosinase expression and melanogenesis and emphasize the critical role of microphthalmia as signal transducer in cAMP-induced melanogenesis and pigment cell differentiation.
|
9700169 |
1998 |
|
Microphthalmos
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The tyrosinase promoter is a principle target of the melanocyte transcription factor Microphthalmia (Mi), a factor for which deficiency in humans causes Waardenburg syndrome II.
|
9830058 |
1998 |
|
Microphthalmos
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Repression correlated with Brn-2/N-Oct3 binding in a mutually exclusive fashion with basic-helix-loop-helix-leucine-zipper (bHLH-LZ) transcription factor USF in vitro and with Brn-2 expression preventing activation of the tyrosinase promoter by the bHLH-LZ factor Microphthalmia in vivo.
|
7478537 |
1995 |