Increased mortality, higher malformation rate, decreased hatching rate, and a shorter total body length, as well as up-regulated cyp19b, vtg1, vtg2, p53, casp3, and casp9 mRNA and down-regulated sod1 and sod2 mRNA were detected in F1 embryos from the F0 and F1 exposure group at 20 and 200 μg/L azoxystrobin (F0+/F1+) when compared with the group from the F0 exposure alone (F0+/F1-).
The results indicate a cytoprotective role for HSP70, regulating caspase-3-mediated apoptosis during embryo development of P. lineatus; however, this mechanism is not effective in controlling embryo viability and larval malformations.