Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Neuroprotective effects were evaluated in a neurotoxic model induced by 6-hydroxydopamine (6-OHDA) in a human neuroblastoma cell line (SH-SY5Y), while the mechanisms associated to neuroprotection were investigated by the determination of mitochondrial membrane potential, H₂O₂ production, Caspase-3 activity, and by observation of DNA fragmentation.
|
30717087 |
2019 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
In conclusion, curcumin strongly induces modulator effects on TRPM2-mediated Ca<sup>2+</sup> influx caused by ROS and caspase 3 and 9 processes in SH-SY5Y neuroblastoma cells.
|
28569571 |
2019 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Exposure of these NB cell lines to 100 μM of harmine resulted in caspase-3/7 and caspase-9 activation as well as caspase-mediated PARP cleavage and Annexin V-positive stained cells, as early as 24 h after treatment, clearly suggesting apoptosis induction, especially in <i>MYCN</i>-amplified cell lines.
|
29977157 |
2018 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
OGD treatment significantly increased N2A cell death and Caspase-3 activity, and decreased Bcl-2 protein expression.
|
29715465 |
2018 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We have shown that targeting of the <i>Plaur</i> gene in mouse neuroblastoma Neuro 2A cells by CRISPR/Cas9n results in ~60% decrease in cell proliferation (p<0.05), reduction in the number of Ki-67 positive cells, caspase 3 activation and PARP-1 cleavage.
|
30034627 |
2018 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We exposed the human neuroblastoma-derived, SH-SY5Y cells to a relatively high concentration of EtOH (500 mM) for 24 h and evaluated the effects of two concentrations of DON (0.1 and 1.0 μM) on alcohol-induced toxicity and caspase-3, an apoptotic marker.
|
29804239 |
2018 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Bt2cAMP also upregulated miR-665, and miR665 transfection mimicked the effects of Bt2cAMP, including reduced c-MYC and HDAC8 expression, increased caspase 3 activation, and reduced neuroblastoma cell growth.
|
30237861 |
2018 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Consistently, we show that these mutations cause protein aggregation which are recognised by the autophagic pathway in motoneurons and triggered caspase 3 activation leading to apoptosis in neuroblastoma cells.
|
28709447 |
2017 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The oligomer Aβ (5 μM) significantly increased the level of caspase 3 cleavage and has the ability to induce cytotoxicity in human neuroblastoma SK-N-MC cells.
|
28790888 |
2017 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Treating four established NB cells lines (SMS-KCNR, SH-SY5Y, BE(2)-C, CHLA-90) and two primary NB cell lines with Tolcapone for 48 h decreased cell viability in a dose-dependent manner, with IncuCyte imaging and Western blotting indicating that cell death was due to caspase-3-mediated apoptosis.
|
28429453 |
2017 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Activation of caspase 3 was also observed in xenografts of NB in nude mice upon combination of doxorubicin with the specific PI3K inhibitor PI103.
|
26224681 |
2016 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
We have recently shown that thymoquinone (TQ) has a potent cytotoxic effect and induces apoptosis via caspase-3 activation with down-regulation of XIAP in mouse neuroblastoma (Neuro-2a) cells.
|
26745078 |
2015 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
SsnB lowers the cellular level of glutathione (GSH), increases generation of reactive oxygen species and activates the cleavage of caspase-3 whereas co-incubation of a GSH precursor, N-acetylcysteine, along with SsnB attenuates the inhibitory effects of SsnB and increases the neuroblastoma cell viability.
|
24788776 |
2014 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
The present study demonstrated that treatment with 1-10 μg/ml tunicamycin, a potent revulsant of ER stress, drastically induced TXNIP expression accompanied by the generation of cleaved caspase-3 as an indicator of apoptosis in SK-N-SH human neuroblastoma cells.
|
23774476 |
2013 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Here we show that low micromolar concentrations of zonisamide prevented cleavage of caspase-3 and cell death in human dopaminergic SH-SY5Y neuroblastoma cells that were subjected to endoplasmic reticulum stress induced by tunicamycin or 6-hydroxydopamine.
|
21892618 |
2012 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We therefore investigated the role of IRS-1/-2 in glucose-induced caspase-3 activation using human neuroblastoma cells.
|
21354306 |
2011 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We found that doxycycline prevents Aβ fibrillization and favors the generation of smaller, non-amyloid structures that were non-toxic as determined by the lack of caspase 3 activation in a neuroblastoma cell line.
|
21998304 |
2011 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
These data demonstrate that TNF-α promotes FasL expression through NFAT activation in neuroblastoma cells and this event leads to increased apoptosis through independent caspase-3 activation.
|
21298033 |
2011 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Furthermore, the effects of Aβ and TD/GH on LDH release, apoptosis and its relevant gene expression, involving bcl-2 and bax/caspase-3, were observed in a human neuroblastoma cell line (SH-SY5Y).
|
20821386 |
2010 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
The objective of this study is to define apoptosis and necrosis in an in vitro model system of SH-SY5Y cells, and to investigate appropriate defense mechanisms with caspase-3 small interference RNA (siRNA) and necrostatin-1 (Nec-1).
|
20487627 |
2010 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Our recent study showed that xanthoangelol, a major chalcone constituent of the stem exudates of Angelica keiskei, induced caspase-3-dependent apoptosis in neuroblastoma cells.
|
18379052 |
2008 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Neuroblastoma and brain tumor cell lines are particularly sensitive to neocarzinostatin; the sensitivity of brain tumor lines to neocarzinostatin is enhanced by transfection with an expression construct for Bcl-2 and is proportional in transfected cells to the product of the relative contents of Bcl-2 and caspase-3 (r (2) = 0.7).
|
18338171 |
2008 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Stable expression of a constitutively active mutant of Gas (GalphasQL) protected gamma ray-induced apoptosis which was assessed by analysis of the cleavages of PARP, caspase-9, and caspase-3 and cytochrome C release in SH-SY5Y human neuroblastoma cells.
|
18059134 |
2007 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
In contrast to the usual response typically observed in the majority of cell types, P2X(7) in vitro stimulation did not induce caspase-3 activation or apoptosis of neuroblastoma cells but rather supported their proliferation.
|
16424024 |
2006 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Apoptosis but not necrosis was detected at various stages (early: annexin-V; effector: caspase-3) after 24-48 h of incubation with GA, GC, or 3-OH-GA in OLN-93 but not in neuroblastoma or microglia cells.
|
15774829 |
2005 |