Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
A missense mutation was detected at codon 96, GCT (Alanine) to GTT (Valine), of the caspase 8 gene in one of the NB cell lines lacking caspase 8 expression.
|
11466626 |
2001 |
Central neuroblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
Frequent promoter hypermethylation of RASSF1A and CASP8 in neuroblastoma.
|
17064406 |
2006 |
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Caspase 8-null neuroblastoma cells were resistant to death receptor- and doxorubicin-mediated apoptosis, deficits that were corrected by programmed expression of the enzyme.
|
10802708 |
2000 |
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
However, the IFN-gamma restoration of caspase 8 in some NB cells revealed persistent TRAIL resistance in most NB cell lines examined.
|
12615731 |
2003 |
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Furthermore, the methylation pattern of 14.3.3sigma, RASSF1A and of an intragenic segment of CASP8 was significantly different between MYCN amplified and single copy neuroblastoma suggesting a specific role of epigenetic alterations in aggressive neuroblastoma.
|
16044164 |
2005 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The combined data suggest that FOXO3 is activated by 5-azadC treatment and triggers expression of caspase-8 in caspase-8-negative neuroblastoma, which may have important implication for metastasis, therapy, and death resistance of this childhood malignancy.
|
22493319 |
2012 |
Central neuroblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
Epigenetic alterations have been described as well: caspase-8 (CASP8) and RAS-association domain family 1 isoform A (RASSF1A) DNA-methylation are important events for the development and progression of neuroblastoma.
|
21725203 |
2011 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Here we show that four different retinoic acid (RA) derivatives also increase caspase-8 protein expression in neuroblastoma, medulloblastoma and small cell lung carcinoma cell lines.
|
18342014 |
2008 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Caspase-8 levels correlate with the expression of signal transducer and activator of transcription 1 in high-grade but not lower grade neuroblastoma.
|
16886176 |
2006 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
These data indicate that Fas-mediated apoptosis in neuroblastoma cells is mitochondria-dependent and inhibited both at the mitochondrial level and at the level of caspase 8 activation.
|
11406564 |
2001 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Subcellular fractionation analysis revealed caspase-8 activity in both cytosol and plasma membrane fractions in both NB7 reconstituted caspase-8 cell lines, as well the LNCaP prostate cancer cells.
|
23456622 |
2013 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Exogenous interferon-γ upregulated expression of caspase-8 in 3 of 4 neuroblastoma cell lines and increased the contribution of TRAIL to NK cytotoxicity against 2 of the 3 lines; however, relatively little inhibition of cytotoxicity was observed when activated NK cells were treated with an anti-interferon-γ neutralizing antibody.
|
23719242 |
2013 |
Central neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
A missense SNP in exon 10 of the CASP8 gene SNP D302H was associated with worse overall and event-free survival in patients with MYCN-amplified neuroblastoma tumors.
|
25502557 |
2014 |
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
These findings define caspase-8 as a metastasis suppressor gene that, together with integrins, regulates the survival and invasive capacity of neuroblastoma cells.
|
16397500 |
2006 |
Central neuroblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
As frequent methylation of the CASP8 gene has also been reported in neuroblastoma, we investigated whether RASSF1A and CASP8 methylation were independent or related events.
|
11709729 |
2001 |
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Restoration of TRAIL-induced apoptosis in a caspase-8-deficient neuroblastoma cell line by stable re-expression of caspase-8.
|
15033719 |
2003 |
Central neuroblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
However, approximately 20% of the neuroblastoma cell lines with methylated CASP8 alleles are also highly resistant to staurosporine (STS)- and radiation-induced cell death, presumably because cytochrome c is not released from mitochondria.
|
11896617 |
2002 |
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We propose the simultaneous analysis of hypermethylation of APAF1, TMS1 and CASP8 apoptotic genes on primary NB tumour as a good prognostic factor of disease progression.
|
21104989 |
2011 |
Central neuroblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
Subsequently others have also shown that caspase-8 is silenced by methylation in neuroblastoma and peripheral neural ectodermal tumors, and that the caspase-9 regulator Apaf-1 is silenced by methylation in melanoma cell lines and patient samples.
|
11511973 |
2001 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Re-expression of caspase-8 in neuroblastoma cells lacking endogenous caspase-8 expression was found to promote cell adhesion to extracellular matrix and to activate adhesion-dependent signaling pathways, such as the Erk kinase cascade.
|
18089800 |
2007 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Our results show that restoration of active caspase-8 expression in a caspase-8-deficient NB cell line is necessary and sufficient to fully restore TRAIL sensitivity.
|
15094781 |
2004 |
Central neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Contrarily, the presence of the -652 6N Del or the CASP8 302His variant was reported to be an unfavorable prognostic factor in colorectal cancer or neuroblastoma.
|
27507139 |
2016 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The relative caspase 8 activity of NB cells expressing caspase 8 increased with the prolongation of TRAIL action time.
|
19954364 |
2009 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
We established N-myc-downregulated IMR-32 cells using shRNA lentiviral particles targeting N-myc and examined the effect the N-myc inhibition on TRAIL susceptibility in human neuroblastoma IMR-32 cells expressing caspase-8.
|
30724400 |
2019 |
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
The retinoic acid analogue 4HPR, IFN-gamma, and the demethylating agent 5-aza-cytidine activate this promoter in NB cells that lack endogenous caspase-8, indicating that this element may regulate both constitutive and inducible CASP8 expression.
|
15650242 |
2004 |