MTDH was identified as the miR-22 target in OS cells and MTDH-triggered autophagy played a key function in the miR-22-associated chemotherapy sensitivity.
The aim of this study was to prepare an efficient and low-toxic vector which could deliver astrocyte elevated gene-1 (<i>AEG-1</i>) small interfering RNA (siRNA; siAEG-1) into osteosarcoma cells effectively and silence the targeted gene both in vitro and in vivo.
Taken together, these findings suggested that miR‑448 may inhibit osteosarcoma development by targeting AEG‑1, providing a novel candidate miRNA for development of miRNA‑targeted therapies for osteosarcoma.
Astrocyte-elevated gene-1 (AEG-1) was identified as one of the target genes of miR-342-3p in osteosarcoma cells by bioinformatics analysis, dual-luciferase reporter assay, real-time quantitative polymerase chain reaction, and Western blot analysis.
Taken together, our data strongly suggest that AEG-1 plays a crucial role in osteosarcoma progression through MMP-2, and AEG-1 could be a useful biomarker for the prediction of osteosarcoma progression and prognosis.