|
Immunologic Deficiency Syndromes
|
0.100 |
AlteredExpression
|
group |
BEFREE |
Taken together, our data suggest that PMF monocytes induce myelofibrosis-like phenotype in immunodeficient mice and that PMF and normal BM-derived CD14+/CD34- monocytes give rise to megakaryocyte progenitor cells.
|
31569199 |
2019 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
It uses the Plasmatherm system for thawing, followed by CD34 microbead magnetic-activated cell sorting isolation with a cell separation kit (Whole Blood Columns, Miltenyi Biotec). hCD34 + HSPC phenotypes and functionality were assessed in vitro and hematologic reconstitution determined in vivo in immunodeficient mice.
|
31769050 |
2019 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
To determine the extent to which BCP-ALL RT programs mirror or deviate from specific stages of normal human B-cell differentiation, we transplanted immunodeficient mice with quiescent normal human CD34+ cord blood cells and obtained RT signatures of the regenerating B-lineage populations.
|
31698451 |
2019 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
The study was performed using CIEA NOG (NOG) mice engrafted with human CD34+ hematopoietic stem cells (huNOG) and age-matched immunodeficient NOG mice.
|
31211147 |
2019 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
Immunodeficient mice were injected with human CD34-positive cells and administered eltrombopag orally for 14 days (eltro-mice).
|
30059642 |
2019 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
Multipotent colony-forming units and long-term hematopoietic-reconstituting cells in immunodeficient mice were found exclusively in the ESAM(High) fraction of CD34(+)CD38(-) cells.
|
26774386 |
2016 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
Human CD34(+) human cord blood cells were transduced with an FV vector encoding a methylguanine methyltransferase (MGMT)P140K transgene, transplanted into immunodeficient NOD/SCID IL2Rγ(null) mice, and selected in vivo for gene-modified cells.
|
25786870 |
2015 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
Here, we determined that a population of CD34(+) cells isolated from PLC/PRF/5 hepatoma cells (PLC) appears to function as liver cancer stem cells (LCSCs) by forming HLCs in immunodeficient mice with as few as 100 cells.
|
25519836 |
2015 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
Transduction of unstimulated CD34(+) cells with CD133-LV resulted in gene marking of cells with competitive proliferative advantage in vitro and in immunodeficient mice.
|
25189742 |
2015 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
NOD-scid-gamma immunodeficient mice were engrafted either with human CD34+ hematopoietic stem cells (HSC) (Hu-HSC mice) and immunized with alpha3(IV)NC1 collagen containing the Goodpasture epitopes or with nephritis patients' peripheral blood leukocytes (PBL) (Hu-PBL mice).
|
26048777 |
2015 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
Day-7-cultured CD34(+) cells from two of three JMML patients injected intrafemorally into immunodeficient mice stimulated with human GM-CSF after transplantation displayed significant hematopoietic reconstitution.
|
25102944 |
2015 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
In order to understand the precise mechanisms involved in human APL leukemogenesis, we established a humanized in vivo APL model involving retroviral transduction of PML-RARA into CD34(+) hematopoietic cells from human cord blood and transplantation of these cells into immunodeficient mice.
|
25369030 |
2014 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
To study the effect of the drug on MF stem cells (MF-SCs), splenic CD34(+) cells were treated with AZD1480 and transplanted into immunodeficient mice.
|
25193869 |
2014 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
The clinical vector was high titer; transduced 68-70% normal human CD34(+) cells, as determined by colony-forming unit assays and by xenotransplantation in immunodeficient NOD.CB17-Prkdc(scid)/J (nonobese diabetic/severe combined immunodeficiency (NOD/SCID)) and NOD.Cg-Prkdc(scid) Il2rg(tm1Wjl)/SzJ (NOD/SCID gamma (NSG))) mice; and resulted in the production of T cells in vitro from human SCID-X1 CD34(+) cells.
|
22551777 |
2012 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
Intermediate (int) levels of aldehyde dehydrogenase (ALDH) activity reliably distinguished leukemic CD34(+)CD38(-) cells capable of engrafting immunodeficient mice from residual normal hematopoietic stem cells that exhibited relatively higher ALDH activity.
|
22262762 |
2012 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
To address this hypothesis, we used three mouse strains (non-obese diabetic (NOD)/severe-combined immunodeficient (SCID) (N/S); NOD/SCID γ-chain(-/-) (NSG); and Rag2(-/-) γ-chain(-/-) (DKO)) and two humanization techniques (bone marrow liver thymus (BLT) and human CD34(+) cell bone marrow transplant of newborn mice (hu)) to generate four common types of humanized mice: N/S-BLT, NSG-BLT, NSG-hu, and DKO-hu mice.
|
22569301 |
2012 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
In vivo, xenotransplantation experiments demonstrated that the homing and engraftment capacity of AML blasts and CD34+CD38- cells to immunodeficient mice BM was significantly inhibited by pretreatment with nucleotides.
|
22053107 |
2012 |
|
Immunologic Deficiency Syndromes
|
0.100 |
AlteredExpression
|
group |
BEFREE |
The CD34(+)CD38(-) CML cell population with high aldehyde dehydrogenase (ALDH) activity was the most enriched for immunodeficient mouse engrafting capacity.
|
21132730 |
2011 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
Stem cells of acute myeloid leukemia (AML) have been identified as immunodeficient mouse-repopulating cells with a Lin(-)CD34(+)38(-) phenotype similar to normal hematopoietic stem cells.
|
20028384 |
2010 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
Pretreatment of human CD34(+) cells with sJAM-A-Fc resulted in increased neointima formation 3 weeks after endothelial denudation in the carotid arteries of nonobese diabetic/severe combined immunodeficient mice.
|
20378847 |
2010 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
Furthermore, in vitro-generated CD34(+)CD7(++) progenitors effectively engrafted the thymus of immunodeficient mice, which was enhanced by the addition of an IL-7/IL-7 antibody complex.
|
19491395 |
2009 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
Lin(-)CD34(-) cells showed hematopoietic cell engraftment rate in 2 immunodeficient mouse strains similar to Lin-CD34(+) cells, whereas endothelial cell engraftment was significantly higher.
|
19855080 |
2009 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
We investigated strains of immunodeficient mice bearing human tissue grafts (skin and artery) inoculated with 1 x 10(6) human CD34+ adult hematopoietic stem cells, peripheral blood monuclear cells autologous to the CD34 donor, or both for human cell engraftment.
|
19155972 |
2009 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
The present study demonstrates that cells derived from human CD34(+) cells, isolated from either cord blood or peripheral blood, migrate into the brain after infusion into nonobese diabetic/severe combined immunodeficient mice.
|
14990803 |
2004 |
|
Immunologic Deficiency Syndromes
|
0.100 |
Biomarker
|
group |
BEFREE |
To explore this potential, CD34(+) or highly purified CD34(+)CD38(-)CD7(-) human hematopoietic stem cells from umbilical cord blood and bone marrow were transplanted into immunodeficient mice.
|
12560238 |
2003 |