The expressions of CD24, B7-H4 and PCNA in ovarian cancer tissues with lymph node metastasis were significantly increased compared with those in ovarian cancer tissues without lymph node metastasis (p<0.05).
Evaluation of the explored the role parameters of patients indicated that CD24 expression was negatively associated with age, histological type and lymph node metastasis (p>0.05), but was positively associated with the clinical stage and pathological grading (p<0.05).The <i>in vitro</i> results indicated that the activator (sonic hedgehog, Shh) and inhibitor (GANT61) of Hh signalling significantly enhanced and reduced CD24 expression, respectively, at both the gene and protein levels (p<0.05).The addition of Shh significantly enhanced cellular migration and invasion of SKOV3 cells <i>in vitro</i> (p<0.05) Down regulation of CD24 using siRNA inhibited the tumour-promoting effects of Shh, and the <i>in vivo</i> results confirmed that GANT61 significantly inhibited CD24 expression and reduced tumour growth (p<0.01).
Importantly, multivariate analysis that included tumour size, lymph node metastasis and chemotherapy demonstrated that high CD24 expression is independently associated with poorer survival in luminal A and triple-negative breast cancer (TNBC) subtypes.
We investigated the distribution of CD133, CD44 and CD24 using histological sections and tissue microarrays (TMAs) of human colon adenocarcinoma obtained from patients with and without lymph node metastases and/or liver metastases.