|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Since DNA damage is a first incident occurred during a tumour attack, it is rational that histone H2A.X phosphorylation on tyrosine 39 (H2A.X<sup>Y39ph</sup>) may act as a tumour-relevant factor.
|
31736361 |
2019 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Tumor growth delay and γ-H2AX phosphorylation were measured, and immune response was assessed by immunofluorescence and flow cytometry at 1 and 7 days after radiation therapy.
|
30342090 |
2019 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Based on immunohistochemical staining, the tumor xenografts in mice treated with 29dL showed time-dependent decreases in the intensity of CD31, a marker of blood vessels, whereas the intensity of γ-H2AX, a marker of DNA damage, increased.
|
30776229 |
2019 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
As a preliminary study, cellular damage via γ-H2AX immunofluorescence staining in an irradiated mouse tumor model was used to verify the beam range in vivo.
|
31075786 |
2019 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
The positive rates of H2AX were 26.0% and 2.6% in tumor tissue and adjacent normal tissues, respectively.
|
30920061 |
2019 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
In addition, it reduced post-in vitro proliferation and suppression of tumor growth by inducing the expression of caspase-3 and phospho-H2A.X (Ser139) while reducing the expression of COX-2 in both murine cancer models.
|
31092422 |
2019 |
|
Neoplasms
|
0.100 |
GeneticVariation
|
group |
BEFREE |
Similarly, there were no tumor regions (for uniform irradiation with 12 Gy) with the low levels of γ-H2AX expected for a condition of cycling hypoxia.
|
29746214 |
2018 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
Compared with other treatments, PDMP was most effective in prolonging survival time (<i>P</i> < 0.05), inhibiting tumor growth (<i>P</i> < 0.05), decreasing expression of CD133 (<i>P</i> < 0.05), CD31 (<i>P</i> < 0.05), and aldehyde dehydrogenase 1 (ALDH1) (<i>P</i> > 0.05), inducing G1 phase arrest (<i>P</i> < 0.05), increasing the apoptosis rate (<i>P</i> < 0.05), and in expressing ATM and γ-H2AX (<i>P</i> < 0.05).
|
29416613 |
2018 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
While γ-H2AX fluorescence intensities returned to the background level in the brain 11 days after treatment, the residual γ-H2AX phosphorylation in the radiated tumors remained elevated compared to un-irradiated contralateral tumors.
|
29859114 |
2018 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
DNA damage response biomarkers including proteins involved in DNA damage (δ-H2AX), homologous recombination (RAD51), regulators of error-prone Non Homologous End-Joining (DNA-pk, FANCD2), and PARP-1 were evaluated in 116 high-risk tumors by immunohistochemistry.
|
29955143 |
2018 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Markers of generic and oxidative DNA damage [phosphorylated histone H2AX (γH2AX) and 8-hydroxy-2'-deoxyguanosine (8-OHdG)] were significantly higher in liver metastases compared with their corresponding primary tumors.
|
30031728 |
2018 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
PDMP + radiotherapy treatment was most effective in inhibiting tumor growth, prolonging survival time, decreasing expression of CD31, CD133, and aldehyde dehydrogenase 1 (ALDH1), inducing G2/M phase arrest, apoptosis, and expression of Ataxia telangiectasia mutated (ATM) and histone H2AX phosphorylation (γ-H2AX).
|
28797171 |
2017 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Kinetics of DNA damage after radiotherapy was assessed in eight dogs using repeated in vivo samples of tumor and co-irradiated normal tissue analyzed with comet assay and phosphorylated H2AX (γH2AX) immunohistochemistry.
|
28587165 |
2017 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
We also provide suggestions on how to combine staining against γ-H2AX with stainings against components of the tumour microenvironment, such as hypoxia and blood vessels.
|
27325258 |
2017 |
|
Neoplasms
|
0.100 |
PosttranslationalModification
|
group |
BEFREE |
We have found that a treatment with a low dose (75 nM) of MK-1775, a recently described specific chemical inhibitor of Wee1, decreases CDDP-induced H2AX phosphorylation in p53-negative cells and enhances the Wip1-sensitization of p53-negative tumors.
|
27077811 |
2016 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Increased phosphorylation of H2A.X at Tyr39 was observed in multiple cancer cell lines, and we found that H2AX Tyr39 phosphorylation positively correlated with histological grade, tumor size and tumor node metastasis stage, and negatively correlated with survival.
|
27813335 |
2016 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
Constitutive γH2AX expression correlated with c-MYC levels and DDR activation, and defined a subset of tumors characterised by poor outcome.
|
25544753 |
2015 |
|
Neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
We report here that increased γ-H2AX expression in HCC is associated with tumor size, vascular invasion, TNM stage and reduced survival rate after liver transplantation (LT).
|
25537504 |
2015 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
The DDR signaling as evaluated by phospho-epitope-specific antibodies against Ser139-phosphorylated H2A histone family member X (γH2AX), ataxia telangiectasia mutated (ATM), and ATM- and Rad3-related (ATR) was commonly activated in tumors with both moderate and high extent of accumulated genomic aberrations, the latter tumors showing a more frequent loss of ATM expression.
|
22926521 |
2013 |
|
Neoplasms
|
0.100 |
PosttranslationalModification
|
group |
BEFREE |
Importantly, preliminary data with nude mice xenografted with LNCaP cells showed that (S)-2 prompted a decrease in the tumor volume and an increase in H2AX phosphorylation within the cancer cells.
|
23469273 |
2013 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
BEZ235 treatment induced apoptotic tumor regressions in vivo that correlated with suppression of mTORC1-regulated substrates and reduced H2AX phosphorylation and also with feedback phosphorylation of AKT.
|
23403624 |
2013 |
|
Neoplasms
|
0.100 |
GeneticVariation
|
group |
BEFREE |
Genetic variants in H2AFX may influence risk of non-Hodgkin lymphoma (NHL), a heterogeneous group of lymphoid tumors that are characterized by chromosomal translocations.
|
24069324 |
2013 |
|
Neoplasms
|
0.100 |
PosttranslationalModification
|
group |
BEFREE |
Both near-infrared and doxorubicin inhibited the tumor growth of MDA-MB435 melanoma cell xenografts in nude mice and increased the phosphorylation of p53 at Ser(15), Chk1 at Ser(317), SMC1 at Ser(966), and H2AX at Ser(139) compared with control mice.
|
22515193 |
2012 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Like p53, γ-H2AX staining was significantly associated with advanced tumor stage (p=0.04), histological grade (p<0.0001), histological type (p<0.0001), and vascular space involvement (p=0.05), but not with lymph node involvement (p=0.64) and patients' age (p=0.36).
|
21185067 |
2011 |
|
Neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Resveratrol (50 mg/kg body weight) treatment also inhibited FaDu tumor growth in nude mice, and γ-H2AX and cleaved caspase-3 were strongly increased in xenografts from resveratrol-treated mice compared with controls.
|
21705453 |
2011 |