|
Pemphigus Vulgaris
|
0.300 |
AlteredExpression
|
disease |
BEFREE |
The present study was conducted to assess the relationship between salivary and serum Dsg1 and Dsg3 levels, and whether salivary Dsg1 and Dsg3 levels correlate with clinical disease severity of oral mucosal lesions in PV.
|
28543318 |
2017 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
Even though, the use of saliva anti-DSG3 and anti-DSG1 ELISA for the diagnosis of PV has been already reported, there are no studies concerning the utilization of saliva by the BIOCHIP approach.
|
28064226 |
2017 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
Anti-desmoglein (Dsg) 1 and -Dsg3 antibody titers have an established role in the diagnosis of the autoimmune blistering skin disease pemphigus vulgaris (PV).
|
28300855 |
2017 |
|
Pemphigus Vulgaris
|
0.300 |
AlteredExpression
|
disease |
BEFREE |
Topical hydrocortisone, rapamycin, or Stat3 inhibitor XVIII prevents autoantibody-induced blistering in the PV passive transfer mouse model, correlating with increased epidermal DSG3 expression and decreased phospho-S727 Stat3.
|
28469076 |
2017 |
|
Pemphigus Vulgaris
|
0.300 |
AlteredExpression
|
disease |
BEFREE |
Naringenin also down-regulated the expression of Dsg1, Dsg3 and E-cadherin compared with the PV group.
|
28591691 |
2017 |
|
Pemphigus Vulgaris
|
0.300 |
AlteredExpression
|
disease |
BEFREE |
Interestingly, we found a middle-aged woman with severe disease with circulating anti-Dsg3 antibodies at high levels, typical of pemphigus vulgaris.
|
26567062 |
2016 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
HLA-DR and -DQ genotyping and profiling of antibodies against desmogleins (Dsg) 1 and Dsg3 were performed in ten members of a family including monozygotic twins affected by PV.
|
27177496 |
2016 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
In the absence of flotillins, desmoglein-3 shows an altered localisation pattern in the cell-cell junctions of keratinocytes, which is highly similar to the localisation observed upon treatment with pemphigus vulgaris autoantibodies.
|
27346727 |
2016 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
Dsg3-reactive T regulatory cells (Treg) have been previously identified in PV and healthy carriers of PV-associated HLA class II alleles.
|
26661498 |
2016 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
Finally, adoptive transfer of splenocytes from Dsg3-knockout mice immunized with murine Dsg3 into immunodeficient mice phenotypically recapitulates PV.
|
26597100 |
2016 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
These findings suggest that the Dsg3 epitopes targeted by pathogenic mPV IgG are human specific.
|
25695683 |
2015 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
We demonstrate that Pkp3 becomes tyrosine phosphorylated as early as 30 min upon binding of PV IgG to keratinocyte surface and eventually detaches from its binding partner desmoglein 3 (Dsg3).
|
24328683 |
2014 |
|
Pemphigus Vulgaris
|
0.300 |
AlteredExpression
|
disease |
BEFREE |
Small-molecule inhibition of MK2 and silencing of MK2 expression block PV mAb-induced Dsg3 endocytosis in human keratinocytes.
|
23657501 |
2014 |
|
Pemphigus Vulgaris
|
0.300 |
GeneticVariation
|
disease |
BEFREE |
Depletion of Dsg3 was inhibited by DP-S2849G-GFP in the cytoskeletal (Triton X-100 insoluble) fraction, and keratin filament retraction, a hallmark of PV, was efficiently blocked similar to treatment with the PKC inhibitor Bim-X.
|
25010392 |
2014 |
|
Pemphigus Vulgaris
|
0.300 |
GeneticVariation
|
disease |
BEFREE |
Our data suggest that VH1-46 autoantibody gene usage is commonly found in PV because VH1-46 Abs require few to no mutations to acquire Dsg3 autoreactivity, which may favour their early selection.
|
24942562 |
2014 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
Because loss of cell cohesion and Dsg3 depletion is observed in the autoantibody-mediated blistering skin disease pemphigus vulgaris (PV), we applied antibody fractions of PV patients.
|
24711455 |
2014 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
We show that CD4(+) T cells recognizing immunodominant Dsg3 epitopes in the context of the PV-associated HLA-DRB1*04:02 induce the secretion of Dsg3-specific IgG in vivo.
|
25252957 |
2014 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
These observations allow us to propose a concept for pemphigus blistering disorders as a "desmosome-remodeling impairment disease" involving a mechanism of Dsg3 nonassembly and depletion from desmosomes through PV immunoglobulin G-activated intracellular signaling events.
|
23257250 |
2013 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
Cloning of the DSG3 gene, generation DSG3 knock-out mice and isolation of monoclonal anti-Dsg3 IgG have aided to clarify the pathogenic mechanisms of PV, which are in part dependent on the fate of desmosomal molecules.
|
23368972 |
2013 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
Specific Human Leukocyte Antigen Class II (HLA II) molecules associated with pemphigus vulgaris (PV), mucous membraine pemphigoid (MMP), and mixed connective tissue disease (MCTD) may react with multiple T cell epitopes within desmoglein 3 (Dsg 3), bullous pemphigoid antigen 2 (BPAG 2), and 70 kDa polypeptide small nuclear ribonucleoproteins (snRNP70) in autoantibody production.
|
22053914 |
2012 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
It is well established that autoantibodies against desmoglein 3 and desmoglein 1 (Dsg1) are relevant in the pathogenesis of pemphigus vulgaris and pemphigus foliaceus, including its endemic form fogo selvagem (FS).
|
22810308 |
2012 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
These findings identify the cis-adhesive interface of DSG3 as the immunodominant region targeted by pathogenic antibodies in PV and indicate that autoreactivity relies on somatic mutations generated in the response to an antigen unrelated to DSG3.
|
22996451 |
2012 |
|
Pemphigus Vulgaris
|
0.300 |
AlteredExpression
|
disease |
BEFREE |
Along with the reduced expression of Dsg3, loss and disruption of E-cadherin and a concomitant decreased pSrc signalling was identified in the basal keratinocytes surrounding the blisters in PV.
|
22294297 |
2012 |
|
Pemphigus Vulgaris
|
0.300 |
AlteredExpression
|
disease |
BEFREE |
Four hundred forty-eight grossly tumor-negative lymph nodes were evaluated for expression of 3 markers [PVA (pemphigus vulgaris antigen), PTHrP (parathyroid hormone-related protein), and TACSTD1 (tumor-associated calcium signal transducer 1)].
|
21355082 |
2011 |
|
Pemphigus Vulgaris
|
0.300 |
Biomarker
|
disease |
BEFREE |
In conclusion, the use of the ectodomains of Dsg 3 and 1 as target antigens expressed in a human cell line resulted in sensitive and specific ELISA systems for both diagnosis and monitoring of PV and PF.
|
20163452 |
2010 |